Neuropathic pain enhances expression of HCN2 channel in rat cerebrospinal fluid-contacting nucleus.
- Author:
Tong WU
1
;
Jing CAO
;
Li-Cai ZHANG
Author Information
1. Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China. lczhang@xzmc.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels;
metabolism;
Neuralgia;
metabolism;
Neurons;
metabolism;
Pain Threshold;
Potassium Channels;
metabolism;
Proto-Oncogene Proteins c-fos;
metabolism;
Pyrimidines;
pharmacology;
Rats;
Rats, Sprague-Dawley
- From:
Acta Physiologica Sinica
2014;66(3):323-331
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this research is to explore the distribution and expression of hyperpolarization-activated cyclic nucleotide-gated channels subtype 2 (HCN2) in cerebrospinal fluid (CSF)-contacting nucleus in neuropathic pain, and provide experimental evidence to reveal the biological function and regulation mechanisms of CSF-contacting nucleus in neuropathic pain. Neuropathic pain model was produced by chronic constriction injury (CCI) in Sprague-Dawley (SD) rats. Intracerebroventricular injection of cholera toxin subunit B (CTb) labeled with horseradish peroxidase (CB-HRP) was used to specifically mark distal CSF-contacting nucleus. The thermal withdrawal latency and mechanical withdrawal threshold of rats were recorded to detect the change of pain threshold. The expressions HCN2 channel and c-Fos proteins in CSF-contacting nucleus were detected by immunofluorescence and Western blot. The results showed that, compared with the control group, CTb-treated rats did not show any differences in the expressions of HCN2 channel and c-Fos proteins in CSF-contacting nucleus, as well as pain threshold. At 7, 14 d after CCI operation, the model rats showed not only significantly increased expressions of HCN2 channel and c-Fos in CSF-contacting nucleus, but also decreased pain threshold. ZD7288, a HCN2 channel blocker, could reverse the above changes in neuropathic pain model rats. These results suggest that the CSF-contacting nucleus may be involved in the process of neuropathic pain via the HCN2 channel.
