Upregulation of P2X3 receptors in dorsal root ganglion of TRPV1 knockout female mice.
- Author:
Xiao FANG
1
,
2
;
Xiao-Han SHI
;
Li-Bin HUANG
;
Wei-Fang RONG
;
Bei MA
Author Information
1. Department of Physiology, Secondary Military Medical University, Shanghai 200433, China
2. Department of Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. mabei08@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
Ganglia, Spinal;
metabolism;
Mice;
Mice, Knockout;
Pain;
metabolism;
Pain Threshold;
Proto-Oncogene Proteins c-fos;
metabolism;
Receptors, Purinergic P2X3;
metabolism;
Spinal Cord;
metabolism;
TRPV Cation Channels;
genetics;
Up-Regulation
- From:
Acta Physiologica Sinica
2014;66(4):431-437
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the changes in mechanical pain threshold in the condition of chronic inflammatory pain after transient receptor potential vanilloid 1 (TRPV1) gene was knockout. Hind-paw intraplantar injection of complete freund's adjuvant (CFA, 20 μL) produced peripheral inflammation in wild-type and TRPV1 knockout female mice. The mechanical pain thresholds were measured during the 8 days after injection and pre-injection by using Von-Frey hair. Nine days after injection, mice were killed and the differences of expression of c-Fos and P2X3 receptor in the dorsal root ganglia (DRG) and spinal cord dorsal horn were examined by Western blotting between the two groups. Compared with that in wild-type mice, the mechanical pain threshold was increased significantly in TRPV1 knockout mice (P < 0.05); 3 days after CFA injection, the baseline mechanical pain threshold in the TRPV1 knockout mice group was significantly higher than that in the wild-type mice group (P < 0.05); The result of Western blotting showed that the expression of c-Fos protein both in DRG and spinal cord dorsal horn of TRPV1 knockout mice group was decreased significantly compared with that in wild-type mice group (P < 0.01, P < 0.05), while the expression of P2X3 receptor in DRG of TRPV1 knockout mice group was increased significantly compared with that in wild-type mice group (P < 0.05). Our findings indicate that TRPV1 may influence the peripheral mechanical pain threshold by mediating the expression of c-Fos protein both in DRG and spinal cord dorsal horn and changing the expression of P2X3 receptor in DRG.
