Effect of high-mobility group box 1 on the proliferation of primary neural stem cells in vitro.
- Author:
Man LI
1
,
2
,
3
,
4
;
Yong LUO
;
Yuan LI
;
Lin SUN
Author Information
1. Department of Neurology, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China
2. Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016 , China
3. Basic Medicine College of Shanxi Medical University, Taiyuan 030001, China
4. Department of Orthopedics, Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, China. luoyong1998@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Proliferation;
Cells, Cultured;
HMGB1 Protein;
pharmacology;
JNK Mitogen-Activated Protein Kinases;
metabolism;
Neural Stem Cells;
cytology;
Phosphorylation;
Rats
- From:
Acta Physiologica Sinica
2014;66(4):469-475
- CountryChina
- Language:Chinese
-
Abstract:
The cell counting kit-8 (CCK-8) proliferation assay and diameter measure of neurospheres were used to investigate the effect of high-mobility group box 1 (HMGB1) on proliferation of primary rat neural stem cells (NSCs) in vitro, and c-Jun N-terminal protein kinase (JNK) potent inhibitor SP600125 was used to study the mechanism. The results demonstrated that HMGB1 significantly increased CCK-8 absorbance values and neurosphere diameters at concentrations of 1 and 10 ng/mL at 48 h and 72 h (P < 0.05), and the other HMGB1 concentration groups (0.01, 0.1, 100 ng/mL) showed no significant difference, compared with control group (P > 0.05). HMGB1 at 10 ng/mL significantly increased the NSCs proliferation accompanied by the rising of phosphorylated JNK levels (P < 0.01), and 10 μmol/L SP600125 prevented these effects in HMGB1-cultured NSCs (P < 0.01). In conclusion, low concentration of HMGB1 (1-10 ng/mL) can increase NSCs proliferation, which may play a role by promoting JNK phosphorylation.
