Effect of jianpi tongluo jiedu recipe on expression levels of COX-2, NF-kappaBp65, and Bcl-2 in gastric mucosa of patients with precancerous lesions of gastric cancer.
- Author:
Yu-ning BAI
;
Ping ZHANG
;
Li LI
;
Shao-li WANG
;
Nai-li YAO
;
Run-shun ZHANG
;
Zhen LIU
;
Dong YAN
;
Yu-ling ZHU
;
Ji-zheng MA
;
Yan PENG
;
Ling ZHANG
;
Jian CAO
;
Yan ZHU
;
Wei LIU
- Publication Type:Journal Article
- MeSH: Apoptosis; Cyclooxygenase 2; metabolism; Drugs, Chinese Herbal; pharmacology; therapeutic use; Gastric Mucosa; metabolism; Humans; NF-kappa B; metabolism; Precancerous Conditions; drug therapy; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; Signal Transduction; Stomach Neoplasms; drug therapy; metabolism; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2015;35(2):167-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of Jianpi Tongluo Jiedu Recipe (JTJR) on protein expression levels of COX-2, NF-kappaBp65, Bcl-2, and Bax, mRNA expression levels of COX-2 and Bcl-2, and the apoptotic index (Al) in gastric mucosa of patients with precancerous lesions of gastric cancer (PL-GC).
METHODSTotally 65 PLGC patients were recruited and treated by JTJR (modified by syndrome typing), one dose per day for six successive months. Protein expression levels of COX-2, NF-KBp65, Bcl-2, and Bax were detected in 65 patients using immunohistochemical (IHC) assay before and after treatment. mRNA expression levels of COX-2 and Bcl-2 were detected in 54 patients using reverse transcription-polymerase chain reaction (RT-PCR). Meanwhile, changes of Al was detected in 65 patients using TdT-mediated dUTP-biotin nick end labeling (TUNEL) fluorescence method.
RESULTSAfter treatment with JTJR, positive protein expression levels of COX-2, NF-KBp65, and Bcl-2 were obviously decreased in the gastric mucosa of PLGC patients (P <0.01), but Bax positive protein expression was found to be higher (P < 0.05). At the same time mRNA expression levels of COX-2 and Bcl-2 were significantly lower after treatment than before treatment (P < 0.05, P < 0.01); Al also increased after treatment (P < 0.05).
CONCLUSIONJTJR could promote apoptosis possibly via NF-kappaBp65/COX-2, COX-2/Bcl-2, and NF-kappaBp65/Bcl-2 signaling pathways, thereby affecting PLGC patients.