Expression of PKD1 and PKD2 transcripts and proteins and its significance in different types of kidney tissues and kidney lines.
- Author:
Hai-dan ZHAO
1
;
Cheng-gang XU
;
Chang-lin MEI
;
Tian-mei SUN
;
Yu-Mei WU
;
Xue-Fei SHEN
;
Wen-jing WANG
;
Lin LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Animals; Cell Line; Gene Expression; Humans; Kidney; metabolism; Kidney Tubules, Collecting; metabolism; Kidney Tubules, Distal; metabolism; Kidney Tubules, Proximal; cytology; Polycystic Kidney, Autosomal Dominant; pathology; RNA, Messenger; biosynthesis; genetics; Swine; TRPP Cation Channels; metabolism
- From: Chinese Journal of Pathology 2005;34(10):646-649
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and function of PKD1 and PKD2 in different kidney tissues and cell lines.
METHODSImmunoprecipitation, Western blotting, In situ hybridization and immunohistochemical staining methods were used to observe the expression of PKD1 mRNA and PKD2 mRNA and their protein abundance in different kidney tissues and cell lines.
RESULTSCoordinate expressions of PKD1 and PKD2 were found in all kidney tissues and cell lines. Distribution of PKD1 mRNA and PKD2 mRNA and their protein polycystin-1 and polycystin-2 in normal human adult kidney tissue were mainly expressed in the medullary collecting ducts and distal tubules. Positive staining was also found in the majority of cyst-lining epithelial cells of PKD1 cystic kidney tissue, PKD1 cyst-lining epithelia cell line and LLC-PK1. The expression level of them in cystic epithelia of ADPKD kidney tissue was much higher than that in adult renal tubules (P < 0.01).
CONCLUSIONSSimilar expression pattern of PKD1 and PKD2 and their different tissue distribution in different kidney tissues show that the molecular mutuality of PC-1 and PC-2 might be the base of their functional correlation. Polycystins might play an important role in the maintenance of tubular architecture.
