Research of the preservation of the composite facial allograft.
- Author:
Qin-xiu LIU
1
;
Qing-feng LI
;
Sheng-wu ZHENG
;
Hui-yong WANG
;
Hao JIANG
;
Jiang LI
;
Dan-ning ZHENG
;
Lu-jia TANG
;
Yu-ping LI
;
Di-sheng ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine; Allopurinol; Animals; Dogs; Face; Female; Glutathione; Insulin; Male; Organ Preservation Solutions; Raffinose; Tissue Preservation; methods; Transplantation, Homologous
- From: Chinese Journal of Plastic Surgery 2006;22(5):334-338
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effective method of preserving composite facial allograft so as to attenuate ischemic injury.
METHODSThe composite facial allografts were harvested from dog, perfused and preserved with 4 degrees C physiological sodium chloride and UW solution respectively. Immediately after the removal of the flap, after 12, 24, 48 h of preservation, MTT assay was used to determine the viability of several kinds of tissue, including skin, mucosa, muscle, bleed vessel, nerve and gland. The results of the two groups were compared in term of viability percentage. The pathology of several tissues were observed after 24 and 48 h of storage.
RESULTSThe viability percentage of every tissue conserved in UW solution for 48 hours was more than 75%. There was significant difference between physiological sodium chloride group and UW group (P < 0.05). Some changes, including Porous arrangement of fibers in connective tissue of skin and mucosa, hyalinization of tissue around the hair follicle and edema of cell in hair follicle, enlargement of space between muscle bundles and unclearness of boundary of acinus could be seen in physiological sodium chloride group while no significant change in UW group.
CONCLUSIONSUW solution could be considered as preservation solution for composite facial allograft.
