Establishment of immortal lymphoblastoid cell bank of keloids pedigree.
- Author:
Mei SONG
1
;
Jian-hua GAO
;
Xin YAN
;
Xiao-jun LIU
;
Yang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; B-Lymphocytes; Cell Culture Techniques; Cell Line, Transformed; Child; Child, Preschool; Female; Humans; Keloid; blood; genetics; Lymphocyte Activation; Male; Middle Aged; Tissue Banks; Young Adult
- From: Chinese Journal of Plastic Surgery 2006;22(6):445-447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide perpetual research materials for long term studies by establishing immortal lymphoblastoid cell bank of keloids pedigree.
METHODSThe immortal lymphoblastoid cell lines of keloids pedigree were established by Epstein-Barr virus transformation of peripheral blood B lymphocytes.
RESULTS27 immortal lymphoblastoid cell lines of keloids pedigree were obtained successfully, all of the immortal lymphoblastoid cell lines were successfully revivificated after been frozen in liquid nitrogen.
CONCLUSIONSIt is important to establish immortal lymphoblastoid cell bank of keloids pedigree and provide long-term DNA materials for deep study of keloids in the future.
