Pilot study of using autologous bone marrow stromal cells and coral to repair canine segmental mandibular defects.
- Author:
Jie YUAN
1
;
Guang-peng LIU
;
Gang CHAI
;
Bo LIU
;
Feng XU
;
Lei CUI
;
Wei LIU
;
Yi-lin CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anthozoa; Bone Marrow Cells; cytology; Bone Substitutes; Cell Culture Techniques; Dogs; Mandible; pathology; surgery; Mesenchymal Stromal Cells; cytology; Pilot Projects; Tissue Engineering; Tissue Scaffolds; Transplantation, Autologous
- From: Chinese Journal of Plastic Surgery 2007;23(1):51-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo repair segmental mandibular defects with autologous bone marrow stromal cells (BMSCs) engineered bone.
METHODSIsolated BMSCs were expanded in vitro and osteogenic induced. In 12 canines, a 3 cm segmental mandibular defect at right mandible was created. 6 canine's defects were repaired with cell-scaffold constructs made from induced BMSCs and coral; others were repaired with coral as control. The engineered bone was evaluated by X-ray, CT, Dual Energy X-ray Absorptiometry (DXA), gross and histological examination, and biomechanical test post-operatively.
RESULTSInduced BMSCs grew well on coral scaffold. At 12 weeks, X-ray showed more callus formed in experimental group, while evident scaffold duration in control group. At 32 weeks, gross observation, X-ray and CT demonstrated well bony-union in experimental group, while bony-nonunion in control group. Also DXA revealed significantly higher bone mineral density of experimental group than control group. Histologically, mature bone were commonly observed and there were bony healing in experimental group, while fibrous healing occurred in control group. Biomechanical test revealed no significant difference between experimental group and normal group.
CONCLUSIONSCanine segmental mandibular defects can be repaired with the tissue-engineered bone generated by coral scaffold with autologous osteogenic BMSCs.
