An observation of the effects of the truncated septin2 on mouse epidermal cell and fibroblast.
- Author:
Jun ZHANG
1
;
Sheng-Jian TANG
;
Xiao-Ke WANG
;
Xiao-Qin LIANG
;
Wen-Juan SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cytoskeletal Proteins; genetics; DNA, Recombinant; Epithelial Cells; cytology; Fibroblasts; cytology; GTP-Binding Proteins; genetics; Genetic Vectors; Mice; Mice, Inbred BALB C; Septins; Skin; cytology; Transfection
- From: Chinese Journal of Plastic Surgery 2007;23(2):147-150
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct eukaryotic expression vector of the truncated septin2 and investigate the influence on the cultured mouse epidermal cell and fibroblast in vitro exerted by the transgenic expression product.
METHODSThe short splicing fragment was obtained by amplifying the reverse transcription product of the fetal mouse skin mRNA with PCR. Then its recombinant expression vector pcDNA3.1 (-)/septin2s was constructed and used to transfect the mouse epidermal cell and fibroblast cultured in vitro. The expression of the foreign gene was detected with RT-PCR and the changes of cell proliferation were observed and analysed.
RESULTSRT-PCR results indicated that pcDNA3.1/septin2 was expressed in the cultured mouse epidermal cells and fibroblasts in vitro. We found that the epidermal cells accelerated their reproduction, but the fibroblasts had no obvious changes.
CONCLUSIONWe successfully constructed eukaryotic expressive vectors of pcDNA3.1/ septin2s and transfected it into mouse epidermal cells and fibroblasts in vitro. The results settle a basis for showing effect of septin2s on fetal mouse skin.
