Metabolic characteristics of GS-nS0 myeloma cells producing anti-CD25 monoclonal antibody in serum-free culture.
- Author:
Liang ZHAO
1
;
Li FAN
;
Xu ZHANG
;
Wensong TAN
Author Information
1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal;
biosynthesis;
immunology;
Cell Line, Tumor;
Culture Media, Serum-Free;
pharmacology;
Culture Techniques;
methods;
Humans;
Interleukin-2 Receptor alpha Subunit;
immunology;
Multiple Myeloma;
metabolism;
pathology;
Recombinant Proteins;
biosynthesis;
immunology;
T-Lymphocytes, Regulatory;
immunology
- From:
Chinese Journal of Biotechnology
2009;25(7):1069-1076
- CountryChina
- Language:Chinese
-
Abstract:
As an immunodepressant, anti-CD25 monoclonal antibody has a huge market with wide prospect and economic value. We developed a low protein serum-free medium for large-scale GS-NS0 myeloma cell culture and anti-CD25 monoclonal antibody production. Further study focused on the characteristics of GS-NSO cell growth, glucose and amino acid metabolism, and antibody production. In the serum-free medium, the maximal viable cell density and antibody concentration reached above 3x10(6) cells/mL and 300 mg/L in batch culture. Compared with the commercial serum-free medium (Excell 620 + 0.2% Primatone), the maximal viable cell density doubled and the maximal antibody concentration increased 46%. Results also showed the specific growth rate decreased when the glucose concentration was lower than 6 mmol/L. And the production of lactate increased when glucose concentration was excessively high (> 30 mmol/L). These results were important to provide technique and theory basis for developing optimized GS-NS0 cell culture and anti-CD25 monoclonal antibody production processes.
