Impact of exogenous paraquat on enzyme exudation and biochemical changes of lignin degradation fungi.
- Author:
Yunchen ZHAO
1
;
Jianlong LI
;
Yuru CHEN
;
Haixia HUANG
;
Zui YU
Author Information
1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China.
- Publication Type:Journal Article
- MeSH:
Fungi;
drug effects;
enzymology;
Lignin;
metabolism;
Oxidative Stress;
Paraquat;
pharmacology;
Peroxidases;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(8):1144-1150
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of exogenous oxygen, we added water solution of paraquat to 7 d cultures of Coriolus versicolor for the next 148 h. Enzyme exudation and biochemical process were investigated on the addition of paraquat. We found that compared with the control (without paraquat), the addition of 30 micromol/L paraquat stimulated the activity of manganese dependent peroxidase (MnP), lignin peroxidase (LiP), and laccases (Lac) 7, 2.5 and 1.3 times, respectively. Also, addition of paraquat enhanced activity of superoxide dismutase (SOD) and catalase (CAT) in the first 48 h. Impact of paraquat on ligninolytic enzymes was significant than that on antioxidant enzyme. Addition of paraquat enhanced phenolic compounds and formaldehyde of cultures too. And concentration of malondialdehyde was increased in the first 24 h. The results showed that addition of paraquat promoted oxidative stress, but the antioxidant systems of the fungal strain are sufficient to prevent mycelia from oxidative stress. As exogenous oxygen, paraquat might be a useful substrate in degradation of lignocellulose.
