A novel screening system for Pseudomonas aeruginosa quorum sensing inhibitors.
- Author:
Weijie SUN
1
;
Yuan WANG
;
Lixin SHEN
;
Kangmin DUAN
Author Information
1. Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, China.
- Publication Type:Journal Article
- MeSH:
Acanthaceae;
chemistry;
Anti-Bacterial Agents;
pharmacology;
Drug Evaluation, Preclinical;
methods;
Drugs, Chinese Herbal;
pharmacology;
Genes, Reporter;
Promoter Regions, Genetic;
Pseudomonas aeruginosa;
drug effects;
genetics;
Quorum Sensing;
drug effects;
Valerianaceae;
chemistry
- From:
Chinese Journal of Biotechnology
2009;25(8):1173-1179
- CountryChina
- Language:Chinese
-
Abstract:
Quorum sensing is an important gene regulatory mechanism in Pseudomonas aeruginosa and controls the expression of numerous virulence factors. We designed and constructed a screening system for quorum-sensing inhibitors. We developed the system by using the lasI and rhlA promoters fused with promoterless sacB as reporters. Using this system we screened a number of Chinese herb extracts, and identified three herb extracts containing inhibitors to the quorum-sensing system and to its regulated genes. The screening system developed was highly efficient and sensitive. It could serve as a useful tool to identify herb compounds that block infections but unlikely render antibiotic resistance in pathogens.
