Synthesis and characterization of alginate oligosaccharides produced by Pseudomonas mendocina NK-01.
- Author:
Wenbin GUO
1
;
Shufang WANG
;
Mingfeng CAO
;
Weitao GENG
;
Cunjiang SONG
Author Information
1. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
- Publication Type:Journal Article
- MeSH:
Alginates;
chemistry;
Glucuronic Acid;
biosynthesis;
chemistry;
genetics;
Hexuronic Acids;
chemistry;
Molecular Weight;
Oligosaccharides;
biosynthesis;
chemistry;
genetics;
Pseudomonas mendocina;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(9):1366-1370
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve, medium chain length poly (3-hydroxyalkanoates), it also synthesized extracellular saccharides, alginate oligosaccharides. The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production. We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy, 1H and 13C of Nuclear Magnetic Resonance, and found that it was compounded in line from beta-D-mannuronic acids and alpha-L-gluronic acids via beta-(1-->4)/ alpha-(1-->4) bonds, which acetylated partly on the 2- and/or 3-hydroxy. In addition, we determined the weight-average molecular weight of alginate oligosaccharides by gel permeation chromatography to be 2054.
