Interleukin 24 inhibits growth and induces apoptosis of osteosarcoma cells MG-63 in vitro and in vivo.
- Author:
Yali HAN
1
;
Jingcheng MIAO
;
Weihua SHENG
;
Xiaohua WANG
;
Yingying JING
;
Yunbo SHAN
;
Tielian LIU
;
Wanrong BAO
;
Jicheng YANG
Author Information
1. Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Animals;
Apoptosis;
genetics;
Bone Neoplasms;
pathology;
therapy;
Caspase 3;
metabolism;
Cell Line, Tumor;
Genetic Therapy;
Humans;
Interleukins;
biosynthesis;
genetics;
Mice;
Mice, Nude;
Osteosarcoma;
pathology;
therapy;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Recombinant Proteins;
biosynthesis;
genetics;
bcl-2-Associated X Protein;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(10):1538-1545
- CountryChina
- Language:Chinese
-
Abstract:
To study the inhibitory effect and anti-cancer mechanisms of interleukin 24 (IL-24) on human osteosarcoma cell MG-63, we delivered IL-24 into MG-63 cells in vitro and in vivo by adenovirus. The expression level of IL-24 was detected by RT-PCR and fluorescence microscope; the growth inhibition, apoptosis rate and apoptosis body were measured by MTT, Flow cytometry and Hoechst staining respectively. Furthermore, we analyzed the expression of bcl-2, bax, caspase3 genes by RT-PCR after overexpression of IL-24. For in vivo study, we first established the MG-63 tumor model by grafting MG-63 cells in athymic nude mice; and then injected Ad-IL-24 into the tumors. Two weeks after injection, we sacrificed the mice, removed the tumors, weighed and calculated the ratios of tumor-suppression. We also detected the expressions of Bcl-2, Bax, Caspase-3 and CD34 with immumohistochemistry. Our in vitro results indicated that Ad-IL-24 was transcribed and translated in MG-63 osteosarcoma cells. More interestingly, IL-24 inhibited the growth of MG-63 cells and induced apoptosis by up-regulation of bax, caspase-3 and down-regulation of bcl-2. The in vivo data showed that IL-24 suppressed the tumor growth conspicuously through down-regulating the expression of bcl-2, and up-regulating the expression of bax, caspase-3. This study would provide evidence for the gene therapy of IL-24 on osteosarcoma.
