Adenovirus mediated IL-24 gene expression suppresses gastric cancer cell growth in vitro.
- Author:
Wanrong BAO
1
;
Jingcheng MIAO
;
Weihua SHENG
;
Yunbo SHAN
;
Zhengyi LI
;
Xiaohua WANG
;
Yingying JING
;
Yali HAN
;
Jicheng YANG
Author Information
1. Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Apoptosis;
genetics;
Caspase 3;
genetics;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Interleukins;
biosynthesis;
genetics;
Proto-Oncogene Proteins c-bcl-2;
genetics;
metabolism;
Recombinant Proteins;
biosynthesis;
genetics;
Stomach Neoplasms;
genetics;
pathology;
Transfection;
Tumor Suppressor Protein p53;
genetics;
metabolism;
Up-Regulation;
bcl-2-Associated X Protein;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(10):1586-1592
- CountryChina
- Language:Chinese
-
Abstract:
To study the inhibitory effect of a recombinant adenoviral vector carrying human IL-24 gene on SGC-7901 human gastric cancer cell. We infected the SGC-7901 gastric cancer cells with Ad blank adenovirus at various multiplicity of infection (MOIs) to find the optimal infective dose. The SGC-7901 tumor cells were infected with Ad-IL-24 at the optimal MOI in the following experiments. Adenovirus-mediated IL-24 transcription expression in SGC-7901 cells was examined by RT-PCR. The growth-suppressing effect of Ad-IL-24 on SGC-7901 tumor cells was assessed by MTT assay. Apoptosis and cell cycle of SGC-7901 tumor cells infected with Ad-IL-24 was evaluated by flow cytometer (FCM), respectively. The karyomorphology of apoptotic SGC-7901 tumor cells was examined using Hoechst33258 staining under fluorescence microscopy. The expression of apoptosis-related genes was future determined by semi-quantification RT-PCR; We demonstrated that the MOI of 100 was the optimal infective dose in the study on adenovirus-mediated IL-24 gene transfer into SGC-7901 gastric cancer cell; IL-24 gene mediated by adenovirus could successfully transcribe in SGC-7901 tumor cells; Ad-IL-24 could significantly inhibit SGC-7901 tumor cell growth and induce apoptosis, it also can up-regulate the express of bax, caspase-3 and p53 whilst down-regulate the bcl-2 expression. Thus, adenovirus-mediated IL-24 expression had marked anti-tumor effect in suppressing SGC-7901 human gastric cancer cell growth and inducing apoptosis, which may be closely associated with its up-regulation of bax/bcl-2, caspase-3 and p53.
