Construction and immunogenicity of recombinant adenoviruses expressing Cap protein of PCV2 and GP5 protein of PRRSV in mice.
- Author:
Xianwei WANG
1
;
Yufeng LI
;
Ping JIANG
Author Information
1. Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Animals;
Capsid Proteins;
biosynthesis;
genetics;
immunology;
Cell Line;
Circoviridae Infections;
prevention & control;
virology;
Circovirus;
genetics;
metabolism;
Immunization;
Mice;
Porcine Reproductive and Respiratory Syndrome;
prevention & control;
virology;
Porcine respiratory and reproductive syndrome virus;
genetics;
metabolism;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
immunology;
Swine;
Transfection;
Viral Envelope Proteins;
biosynthesis;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2009;25(11):1639-1645
- CountryChina
- Language:Chinese
-
Abstract:
Porcine circovirus 2 (PCV2) has been implicated as the etiological agent of postweaning multisystemic wasting syndrome (PMWS). Co-infection of PCV2 and porcine reproductive and respiratory syndrome virus (PRRSV) can result in severe economic losses to the swine industry. In this study, we constructed the recombinant adenovirus rAd-Cap-GP5 expressing Cap of PCV2 and GP5 of PRRSV. And the expression of Cap and GP5 protein in the HEK-293 cells inoculated with rAd-Cap-GP5 were confirmed by immunoperoxidase monolayer assay (IPMA), indirect immunofluorescence assay (IFA) and Western blotting, respectively. The immunogenicity of recombinant adenoviruses rAd-Cap-GP5 was examined in mice by vaccination with the recombinant adenovirus. The results showed that the mice could produce anti-PCV2 and PRRSV antibodies detected by indirect ELISA and virus neutralization assay. It indicated that rAd-Cap-GP5 could provide humoral immunity responses in mice. The recombinant adenovirus rAd-Cap-GP5 might be an attractive candidate vaccine for preventing the disease associated with PCV2 and PRRSV infection.
