DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene.
- Author:
Yanwei CUI
1
;
Zhifeng ZENG
;
Hongjian LI
;
Yueqin LI
;
Qi ZHOU
;
Dan YANG
;
Yi ZOU
;
Guang YANG
;
Tianhong ZHOU
Author Information
1. National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cytomegalovirus;
drug effects;
genetics;
metabolism;
Cytomegalovirus Infections;
enzymology;
virology;
DNA, Viral;
genetics;
Directed Molecular Evolution;
methods;
Gene Expression Regulation, Viral;
Humans;
Nucleic Acid Conformation;
Oligodeoxyribonucleotides;
genetics;
pharmacology;
RNA, Guide;
chemistry;
pharmacology;
RNA, Messenger;
genetics;
metabolism;
Ribonuclease P;
genetics;
metabolism;
Viral Structural Proteins;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(11):1690-1696
- CountryChina
- Language:Chinese
-
Abstract:
External Guide Sequences (EGSs) represents a novel nucleic acid based gene interference approach to modulate gene expression. They are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. DNA-based EGS1386 with a size of 12 nt was chemically synthesized to target the mRNA coding for the UL49 gene of human cytomegalovirus (HCMV). The DNA-based EGS1386 molecule efficiently directed human RNase P to cleave the target mRNA sequence in vitro. A reduction of more than 50% in the levels of UL49 expression was observed in human cells treated with the DNA-based EGS1386 targeted UL49 assayed by fluorescent quantization PCR and Western blotting. This results showed that the DNA-EGS1386 can effectively guide the RNase P cut the target mRNA. Therefore, DNA-EGS can develop into a new gene silencing technology and potential of the anti-viral reagents.
