Effects of surfactin on proliferation, apoptosis and cytoskeleton in human breast cancer MCF-7 cells.
- Author:
Xiaohong CAO
1
;
Aihua WANG
;
Chunling WANG
;
Meifang LU
;
Runzhi JIAO
;
Hui ZHU
;
Sisi ZHAO
Author Information
1. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. wahwawayu@126.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Breast Neoplasms;
pathology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cytoskeleton;
drug effects;
Female;
Humans;
Lipopeptides;
pharmacology;
Peptides, Cyclic;
pharmacology
- From:
Chinese Journal of Biotechnology
2009;25(11):1705-1710
- CountryChina
- Language:Chinese
-
Abstract:
We studied the effect of surfactin on cell proliferation, apoptosis and the cytoskeleton in human breast cancer cell line MCF-7 in vitro. The result of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed that the surfactin inhibited proliferation of MCF-7 cells in a dose- and time-dependent manner, with IC50 at 48 h of 27.3 micromol/L. Surfactin-induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes by AO/EB staining. Flow cytometric analysis also demonstrated that surfactin caused time-dependent apoptosis of MCF-7 cells through cell arrest at G2/M phase. Immunofluorescence and Western blotting showed that surfactin significantly suppressed the expression of vimentin, induced the alpha-tubulin depolymerization and rearrangement and then the skeleton system of the cells changed dramatically. Based on our findings, surfactin can significantly inhibit the growth of MCF-7 cells and induce apoptosis.
