High-yielding cordycepin in Cordyceps militaris modified by low-energy ion beam.
- Author:
Wen LI
1
;
Shiguang ZHAO
;
Hongwei CHEN
;
Hang YUAN
;
Tao WANG
;
Xian HUANG
Author Information
1. College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China.
- Publication Type:Journal Article
- MeSH:
Cordyceps;
growth & development;
metabolism;
radiation effects;
Culture Media;
Culture Techniques;
methods;
Deoxyadenosines;
biosynthesis;
isolation & purification;
radiation effects;
Ions;
Linear Energy Transfer
- From:
Chinese Journal of Biotechnology
2009;25(11):1725-1731
- CountryChina
- Language:Chinese
-
Abstract:
Cordycepin has the effect of anti-tumor, immunomodulation, anti-inflammation and so on. In order to make use of Cordyceps militaris better, we implanted different doses of low-energy ion beam into C. militaris, chose best cordycepin extracting technology, and determined cordycepin content in strains before and after ion beam implantation by UV spectrophotometry. Results showed that the best dose of low-energy ion beam was 2.60 x 10(15) ions/cm2, the best conditions of microwave assisted ultrasonic extraction technology were as follows: 70% ethanol as the solvent, microwave power as 200 W, extraction time as 110 s, Material - liquid ratio as 1: 240. We chose 15 strains with high-yielding cordycepin, of which cordycepin content was up to (11.924 +/- 0.063) mg/g, which was a nearly 30% increase compared with the original strain.
