Recent advances in enzyme assays using fluoremetry.
- Author:
Yanlong XING
1
;
Xiangzhao MAO
;
Shu WANG
;
Hualei WANG
;
Dongzhi WEI
Author Information
1. Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Science, Qingdao 266101, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Enzyme Assays;
methods;
trends;
Fluorescent Dyes;
Fluorometry;
methods;
Humans;
Microscopy, Fluorescence
- From:
Chinese Journal of Biotechnology
2009;25(12):1765-1769
- CountryChina
- Language:Chinese
-
Abstract:
Enzymes play such a pivotal role in cellular metabolism that enzyme assays are important for bio-engineering, disease diagnoses and drug discovery. Among the reported methods, fluoremetry has attracted more and more attention due to its high sensitivity and possibility of continuous dynamic monitoring. The recent progresses and applications in enzyme assays using fluorescent probes were reviewed. Different methods were classified into direct fluorescence detection and indirect fluorescence detection according to their labeled substrates and detection mechanisms. Our writing purpose is to provide the readers with a flavor of the kinds of tools and strategies available in enzyme assays with fluorescent probes. Also, the research situation and prospects were disucssed
