Correlation between epigenetic alterations in the insulin growth factor-II gene and hepatocellular carcinoma.
10.3760/cma.j.issn.1007-3418.2012.08.011
- Author:
Zhi-zhen DONG
1
;
Deng-fu YAO
;
Wei WU
;
Li-wei QIU
;
Ning-hua YAO
;
Xiao-di YAN
;
Dan-dan YU
;
Jie CHEN
Author Information
1. Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Carcinoma, Hepatocellular;
genetics;
metabolism;
pathology;
CpG Islands;
genetics;
DNA Methylation;
Epigenesis, Genetic;
Gene Expression Regulation, Neoplastic;
Humans;
Immunohistochemistry;
Insulin-Like Growth Factor II;
genetics;
metabolism;
Liver;
metabolism;
pathology;
Liver Neoplasms;
genetics;
metabolism;
pathology;
Middle Aged;
Polymerase Chain Reaction;
methods;
Promoter Regions, Genetic;
RNA, Messenger;
genetics;
metabolism;
Transcription, Genetic
- From:
Chinese Journal of Hepatology
2012;20(8):593-597
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether epigenetic alterations in the insulin-like growth factor-II (IGF-II) gene that cause differential transcription or expression are correlated with onset and severity of hepatocellular carcinoma (HCC). Patient-matched specimens of HCC, paracancerous, and non-cancerous tissues were collected from 40 primary liver cancer patients. Epigenetic alterations in the promoter (P3) sequence of the IGF-II gene were analyzed by methylation-specific PCR (MSP) and IGF-II transcription was measured by RT-PCR. IGF-II protein expression and clinicopathological features were assessed by immunohistochemistry and microscopic observation. The rate of IGF-II P3 methylation was significantly lower in HCC tissues (0%) than in paracancerous tissues (vs. 47.5%; x2 = 24.918, P less than 0.001) and non-cancerous tissues (vs. 100%; x2 = 80.000, P less than 0.001). IGF-II mRNA expression was significantly higher in HCC tissues (100%) than in paracancerous tissues (vs. 52.5%; x2 = 24.918, P less than 0.001) and non-cancerous tissues (vs. 0%; x2 = 80.000, P less than 0.001). IGF-II protein expression was significantly higher in HCC tissues (82.5%) than in paracancerous tissues (vs. 45.0%; x2 = 12.170, P less than 0.001) and non-cancerous tissues (vs. 0%; x2 = 56.170, P less than 0.001). IGF-II overexpression in HCC was significantly associated with degree of differentiation, extent of infiltrated serosa, size of tumor, and HBV-positive infection status. Epigenetic alterations in the IGF-II gene regulate its transcription and expression and are closely associated with HCC development and progression.
