Study on interference therapy induced by epidermal growth factor receptor-antisense cDNA in signal transduction of laryngeal squamous cell carcinoma.
- Author:
Jun-ming XIAN
1
;
Guang-yao ZHOU
;
Chuan-yu LIANG
;
Shi-xi LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; Apoptosis; Carcinoma, Squamous Cell; Cell Cycle; Cell Proliferation; DNA, Complementary; Genetic Vectors; HEK293 Cells; Head and Neck Neoplasms; Humans; Laryngeal Neoplasms; Receptor, Epidermal Growth Factor; Signal Transduction; Transfection
- From: West China Journal of Stomatology 2007;25(6):540-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate effect of interference therapy induced by epidermal growth factor receptor (EGFR)-antisense cDNA in signal transduction of Hep-2 laryngeal squamous cell carcinoma in vitro.
METHODSAdEasy Vector System was used to construct the recombinant adenovirus vector sense/antisense-pAdEasy-EGFR. The recombinant adenovirus vector introduced EGFR-sense/antisense cDNA fragment into HEK293 cell. The purified recombinant adenovirus sense/antisense-pAdEasy-EGFR transfected Hep-2 cells in vitro. The inhibition of EGFR protein expression and proliferation of Hep-2 cells, the changes of cell cycle and DNA content in Hep-2 cells were examined by MTT, Western blot analysis, flow cytometry essay, and immunocytochemistry respectively.
RESULTSThe higher titre sense and antisense mRNA expression recombinant adenovirus containing 1,032 bp EGFR-cDNA was constructed and prepared successfully. When antisense-pAdEasy-EGFR was transferred into Hep-2 cells the inhibition of cell proliferation and EGFR protein expression in Hep-2 cells were investigated effectively.
CONCLUSIONThe antisense-pAdEasy-EGFR effectively interfere the Hep-2 signal transduction pathway and induce apoptosis which results in inhibiting proliferation of laryngeal squamous cell carcinoma.