Effect of TRAIL gene in human squamous cell carcinoma cell line induced by adenovirus.
- Author:
Guang-xiang ZANG
1
;
Ya-bing MU
;
Hong-chen SUN
;
Jin-zhong LIU
;
Ze-bing ZHANG
;
Xiao-liang KE
;
Lei-ying MIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; Apoptosis; Carcinoma, Squamous Cell; Cell Line; Genetic Vectors; Green Fluorescent Proteins; Humans; Promoter Regions, Genetic
- From: West China Journal of Stomatology 2007;25(6):584-587
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the apoptotic effect on the squamous cell carcinoma cell line TCa83 induced by recombined adenovirus vector containing TRAIL gene and CMV promoter.
METHODSThe TCa83 cell line was firstly infected with different titre of AdCMV-EGFP containing enhanced green fluorescence protein gene (EGFP) as control, and investigated the transducing rate through fluorescence to obtain the definite titre. Then TCa83 cell line was infected with AdCMV-TRAIL in proper titre, and TRAIL gene was detected by means of RT-PCR. After TCa83 cell line was infected with AdCMV-TRAIL and AdCMV-EGFP at day 1, 3, 5, 7, the activity of TCa83 cell line were evaluated by MIT and the apoptosis were detected by flow cytometer.
RESULTSProper titre was of 1,000 particles/cell, and TCa83 cell line could be infected 100% in this titre. TRAIL gene was detected by RT-PCR after infected with AdCMV-TRAIL. The activity of TCa83 decreased in both groups, but the AdCMV-TRAIL group decreased more sharply than AdCMV-EGFP group (P < 0.001). Both AdCMV-TRAIL and AdCMV-EGFP could lead to apoptosis of TCa83 cells, but the AdCMV-TRAIL, function stronger than AdCMV-EGFP. Especially there was remarkable statistic difference between two groups (P < 0.0001).
CONCLUSIONAdCMV-TRAIL could effectively decrease the activity of TCa83 cell line and induce apoptosis.
