Effects of 17beta-estradiol on the intracellular calcium of masticatory muscles myoblast in vitro.
- Author:
Xi-jun WANG
1
;
Hong-mei GUO
;
Yan-min WANG
;
Xin-zhu YI
Author Information
- Publication Type:Journal Article
- MeSH: Aniline Compounds; Animals; Calcium; Estradiol; Female; In Vitro Techniques; Masticatory Muscles; Myoblasts; Rats; Rats, Sprague-Dawley; Xanthenes
- From: West China Journal of Stomatology 2007;25(6):611-613
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of 17beta-estradiol on the intracellular calcium of masticatory muscles myoblast.
METHODSMyoblasts from maxillofacial skeletal muscle of one week old female Sprague-Dawley rats were cultured. Fluo-4-AM as the Ca2+ indicator and the laser confocal microscope system were used to observe the effects of estrogen on the cytoplasmic Ca2+ concentration in the normal pH condition and the acid condition (pH = 6.7).
RESULTSIn the normal pH condition, when 17beta-estradiol (10(-9), 10(-8), 10(-7) mol/L) were added to cells cytoplasmic Ca2+ immediately increased then decreased right away, and in the end came into a new Ca2+ homeostasis in the base line. In the acid condition, 17beta-estradiol (10(-9), 10(-8), 10(-7) mol/L) made the cytoplasmic Ca2+ decreased immediately then came into a new Ca2+ homeostasis under the base line.
CONCLUSIONThe results suggest that estrogen may maintain the skeletal cytoplasmic Ca2+ concentration in a lower level and reduce the cytoplasmic Ca2+ accumulation to keep the normal functions of masticatory muscles myoblast.
