Effects of basic fibroblast growth factor on the proliferation of human salivary adenoid cystic carcinoma cell line ACC-2 and extracellular signal-regulated kinase, Cyclin D1, p2waf/cip1 signaling pathway.
- Author:
Lei DING
1
;
Sheng-rong ZHU
;
San-xiang XIE
;
Xiang-bing WU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Carcinoma, Adenoid Cystic; Cell Line; Cell Proliferation; Cyclin D1; Extracellular Signal-Regulated MAP Kinases; Fibroblast Growth Factor 2; Humans
- From: West China Journal of Stomatology 2008;26(2):118-120
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of basic fibroblast growth factor (bFGF) on the proliferation of human salivary adenoid cystic carcinoma (ACC) cell line ACC-2 in vitro.
RESULTSThe effect of ectogenic bFGF on proliferation of ACC-2 was observed by MTT assay. Extracellular signal-regulated kinase (ERK) activity was measured by immuno-precipitation. p-ERK1/2, Cyclin D1 and p21waf/cip1 expression were assessed by Western blot.
RESULTSbFGF could enhance the proliferation of ACC-2. Stimulated by bFGF, the proliferation ratio increased significantly. The intracellular ERK activity, p-ERK1/2 and Cyclin D1 expression were increased, while p21waf/cip1 expression was inhibited by different concentrations of bFGF. The above effects of bFGF could be attenuated by MEK inhibitor U0126.
CONCLUSIONbFGF stimulates the proliferation of ACC-2 in a dose dependent manner. The proliferation effect of bFGF may be due to up-regulating ERK, Cyclin D1 and p21waf/cip1 signaling pathway. This research can help us to explore a new pathogenesis and therapy of the ACC.
