Analysis of pathogenic features and infection status of human parainfluenza virus type 3 among children in Hangzhou.
- Author:
Xin QIAN
1
;
Xinfeng YU
;
Min ZHAO
;
Yu KOU
;
Jun LI
;
Yinyan ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Child; China; epidemiology; Genetic Variation; Humans; Parainfluenza Virus 3, Human; Phylogeny; Real-Time Polymerase Chain Reaction; Respiratory Tract Infections; epidemiology; virology; Respirovirus Infections; epidemiology
- From: Chinese Journal of Preventive Medicine 2016;50(3):255-260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the level of genetic variation of human parainfluenza virus type 3 (HPIV-3), and to describe infection and co-infection characteristics of HPIV-3 in children.
METHODSSingle respiratory samples from 856 pediatric patients with acute respiratory tract infection (ARI) in Hangzhou were collected from December 2009 to March 2013. All samples were screened for HPIV-3 by real-time RT-PCR and followed by HN sequencing and phylogenetic analysis. In all RSV positive specimens, we screened for the other pathogens, and co-infection characteristics were evaluated.
RESULTSA total of 9.6% of 856 samples were positive for HPIV-3, the nucleotide among the strains ranged from 96.9% to 100%. All Hangzhou strains were placed in C3 subgroup based on HN gene analysis. 49% (n=41) of all HPIV-3-positive children with ARI were found to be co-infected with at least one of the other pathogen. The highest co-infection rate of HPIV-3 was with HRV (n=17). Children in the younger groups (≤12 months old) were significantly more prone to be co-infected with other pathogen (χ(2)=4.78, P=0.029). Pneumonia infection rate was significantly higher in the mono-infection group than the co-infection group (χ(2)=3.92, P=0.048).
CONCLUSIONHPIV-3 was an important pathogen in children with ARI in Hangzhou. HN gene variation rate was low, but showed a more local pattern. The co-infections with other respiratory viruses were popular. Except for pneumonia, no significant differences in other clinical presentation between the HPIV-3 mono-infection and co-infection groups were observed.