- Author:
Pi Wei ZHANG
1
;
Chong TIAN
2
;
Fang Yi XU
1
;
Zhuo CHEN
1
;
Raynard BURNSIDE
1
;
Wei Jie YI
1
;
Si Yun XIANG
1
;
Xiao XIE
1
;
Nan Nan WU
1
;
Hui YANG
3
;
Na Na ZHAO
4
;
Xiao Lei YE
4
;
Chen Jiang YING
5
;
Author Information
- Publication Type:Letter
- MeSH: Animals; Autophagy; drug effects; Cattle; Cells, Cultured; Endothelial Cells; drug effects; metabolism; Gene Expression Regulation; drug effects; Glucose; toxicity; Macrolides; pharmacology; Polyphenols; chemistry; pharmacology; Tea; chemistry
- From: Biomedical and Environmental Sciences 2016;29(7):524-528
- CountryChina
- Language:English
- Abstract: Bovine aortic endothelial cells (BAECs) were cultured with high glucose (33 mmol/L), 4 mg/L green tea polyphenols (GTPs) or 4 mg/L GTPs co-treatment with high glucose for 24 h in the presence or absence of Bafilomycin-A1 (BAF). We observed that high glucose increased the accumulation of LC3-II. Treatment with BAF did not further increase the accumulation of LC3-II. Results also showed an increased level of p62 and decreased Beclin-1. However, GTPs showed inversed trends of those proteins. Furthermore, GTPs co-treatment with high glucose decreased the level of LC3-II and a much higher accumulation of LC3-II was observed in the presence of BAF in comparison with high glucose alone. Results also showed a decreased p62 and increased Beclin-1. The results demonstrated that GTPs alleviated autophagy inhibition induced by high glucose, which may be involved in the endothelial protective effects of green tea against hyperglycemia.