Green Tea Polyphenols Alleviate Autophagy Inhibition Induced by High Glucose in Endothelial Cells.

Pi Wei Zhang; Chong Tian; Fang Yi XU; Zhuo Chen; Raynard Burnside; Wei Jie YI; Si Yun Xiang; Xiao Xie; Nan Nan WU; Hui Yang; Na Na Zhao; Xiao Lei YE; Chen Jiang Ying

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Green Tea Polyphenols Alleviate Autophagy Inhibition Induced by High Glucose in Endothelial Cells.

Author: Pi Wei ZHANG ¹ ; Chong TIAN ² ; Fang Yi XU ¹ ; Zhuo CHEN ¹ ; Raynard BURNSIDE ¹ ; Wei Jie YI ¹ ; Si Yun XIANG ¹ ; Xiao XIE ¹ ; Nan Nan WU ¹ ; Hui YANG ³ ; Na Na ZHAO ⁴ ; Xiao Lei YE ⁴ ; Chen Jiang YING ⁵ ;
Author Information

1. Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
2. School of Nursing, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
3. Kecheng People's Hospital, Quzhou 324000, Zhejiang, China.
4. School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
5. Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
Publication Type:Letter
MeSH: Animals; Autophagy; drug effects; Cattle; Cells, Cultured; Endothelial Cells; drug effects; metabolism; Gene Expression Regulation; drug effects; Glucose; toxicity; Macrolides; pharmacology; Polyphenols; chemistry; pharmacology; Tea; chemistry
From: Biomedical and Environmental Sciences 2016;29(7):524-528
CountryChina
Language:English
Abstract: Bovine aortic endothelial cells (BAECs) were cultured with high glucose (33 mmol/L), 4 mg/L green tea polyphenols (GTPs) or 4 mg/L GTPs co-treatment with high glucose for 24 h in the presence or absence of Bafilomycin-A1 (BAF). We observed that high glucose increased the accumulation of LC3-II. Treatment with BAF did not further increase the accumulation of LC3-II. Results also showed an increased level of p62 and decreased Beclin-1. However, GTPs showed inversed trends of those proteins. Furthermore, GTPs co-treatment with high glucose decreased the level of LC3-II and a much higher accumulation of LC3-II was observed in the presence of BAF in comparison with high glucose alone. Results also showed a decreased p62 and increased Beclin-1. The results demonstrated that GTPs alleviated autophagy inhibition induced by high glucose, which may be involved in the endothelial protective effects of green tea against hyperglycemia.