Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction.

Yan Hua Kong; Qi Shi; Na Han; Ling Zhang; Yuan Yuan Zhang; Tong Xin Gao; Chen Chen; You Lin LI

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Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction.

Author: Yan Hua KONG ¹ ; Qi SHI ² ; Na HAN ³ ; Ling ZHANG ¹ ; Yuan Yuan ZHANG ⁴ ; Tong Xin GAO ⁵ ; Chen CHEN ⁴ ; You Lin LI ²
Author Information

1. Beijing University of Chinese Medicine, Beijing 100029, China.
2. Second Department of TCM Pulmonary Disease, The Key Institute of State Administration of Traditional Chinese Medicine (pneumonopathy chronic cough and dyspnea), Beijing Key Laboratory (NO. BZ0321), China-Japan Friendship Hospital, Beijing 100029, China.
3. State Key Laboratory for Infectious Diseases Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Beijing 102206, China
4. Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.
5. Nephrology Department, Aviation General Hospital of China Medical University, Beijing 100012, China.
Publication Type:Journal Article
Keywords: Asthma; Gut microbiota; Recuperating lung decoction
MeSH: Animals; Asthma; drug therapy; immunology; microbiology; Bacteria; classification; genetics; isolation & purification; Drugs, Chinese Herbal; administration & dosage; Gastrointestinal Microbiome; drug effects; Gastrointestinal Tract; immunology; microbiology; Humans; Male; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley
From: Biomedical and Environmental Sciences 2016;29(8):574-583
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate whether recuperating lung decoction (RLD) can modulate the composition of gut microbiota in rats during asthma treatment.
METHODSFifteen Sprague-Dawley rats were divided randomly and equally into control group, model group, dexamethasone (DEX) group, RLD medium-dose group, and RLD high-dose group. The asthma model was established in all groups, except for the control group. The rats in the DEX and RLD groups were treated orally with DEX and RLD, respectively. The rats in the control and model groups were treated orally with 0.9% saline. The intestinal bacterial communities were compared among groups using 16S rRNA gene amplification and 454 pyrosequencing.
RESULTSThe microbial flora differed between the control and model groups, but the flora in the RLD groups was similar to that in the control group. No significant differences were observed between the RLD high-dose and medium-dose groups. RLD treatment resulted in an increase in the level beneficial bacteria in the gut, such as Lactobacillus and Bifidobacterium spp.
CONCLUSIONOral administration of RLD increased the number of intestinal lactic acid-producing bacteria, such as Lactobacillus and Bifidobacterium, in asthma model rats.