Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.
- Author:
Qian ZHOU
1
;
Hai Feng WU
2
;
Yuan WEI
3
;
Wen Li ZHU
1
Author Information
1. Department of Nutrition and Food Hygiene, School of Public Health, Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China.
2. Department of Urology, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China.
3. Department of Obstetrics and Gynaecology, Peking University Third Hospital, Beijing 100191, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Hedgehog Proteins;
genetics;
metabolism;
Kruppel-Like Transcription Factors;
genetics;
metabolism;
Mice;
Nerve Tissue Proteins;
genetics;
metabolism;
Palate;
embryology;
metabolism;
RNA, Small Interfering;
genetics;
metabolism;
Signal Transduction;
Zinc Finger Protein Gli2;
Zinc Finger Protein Gli3
- From:
Biomedical and Environmental Sciences
2016;29(8):594-598
- CountryChina
- Language:English
-
Abstract:
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 μg/mL cyclopamine group was 68.9% lower than that of blank control group (P<0.05). Hedgehog signaling pathway inhibition decreased palatal fusion in organ culture, probably owing to downregulation of Smo and Gli1 proteins.
