Evaluation of Xpert MTB/RIF for the Diagnosis of Extrapulmonary Tuberculosis in China.
- Author:
Mei YUAN
1
,
2
;
Yan LYU
3
;
Su Ting CHEN
4
;
Chao CAI
4
;
Yuan LI
5
;
Zhi Guo ZHANG
6
;
Yun Xu LI
4
;
Ling Ling DONG
4
;
Yu Hong FU
4
;
Hai Rong HUANG
4
;
Ji Min GAO
7
;
Wei Min LI
4
Author Information
1. Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
2. National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
3. Department of Radiology, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
4. National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
5. Department of Orthopaedics, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
6. Institute of Tuberculosis Prevention and Control of District Changping, Beijing 102200, China.
7. Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
- Publication Type:Journal Article
- MeSH:
Bacterial Proteins;
genetics;
metabolism;
China;
DNA-Directed RNA Polymerases;
genetics;
metabolism;
Diagnostic Tests, Routine;
instrumentation;
methods;
Drug Resistance, Bacterial;
Humans;
Microbial Sensitivity Tests;
Mycobacterium tuberculosis;
drug effects;
genetics;
isolation & purification;
metabolism;
Retrospective Studies;
Rifampin;
pharmacology;
Sensitivity and Specificity;
Sputum;
Tuberculosis;
Tuberculosis, Pulmonary;
diagnosis;
microbiology
- From:
Biomedical and Environmental Sciences
2016;29(8):599-602
- CountryChina
- Language:English
-
Abstract:
We evaluate the performance of Xpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis (EPTB) in China. The performance of Xpert was evaluated compared to the composite reference standard (CRS), drug susceptibility testing (DST), and imaging examination. The overall sensitivity and specificity of Xpert were 64.1% (195/304) and 100% (24/24), respectively, using CRS as the gold standard. The sensitivity was significantly higher than that of culture for pus (P<0.05). The proportion of EPTB-positive cases diagnosed by imaging was two times more than that diagnosed using Xpert; however, 6 out of 19 cases may have been overdiagnosed by imaging. Compared to phenotypic DST, the sensitivity and specificity of Xpert were 80% (12/15) and 100% (75/75), respectively. Considering its high sensitivity and specificity, Xpert MTB/RIF may be used as a rapid initial test for EPTB diagnosis, and may also support a quicker decision on the treatment regimen. The combination of imaging and Xpert testing could provide high efficiency and accurate diagnosis of suspected EPTB.
