Long Noncoding RNA Expression Signatures of Abdominal Aortic Aneurysm Revealed by Microarray.

Yao Guo Yang; Mei Xia LI; Lei Kou; You Zhou; Yan Wen Qin; Xiao Jun Liu; Zhong Chen

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Long Noncoding RNA Expression Signatures of Abdominal Aortic Aneurysm Revealed by Microarray.

Author: Yao Guo YANG ¹ ; Mei Xia LI ² ; Lei KOU ¹ ; You ZHOU ¹ ; Yan Wen QIN ¹ ; Xiao Jun LIU ³ ; Zhong CHEN ¹
Author Information

1. The Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
2. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
3. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
Publication Type:Journal Article
Keywords: ALOX5; Abdominal aortic aneurysm; lnc-ARG; lncRNA
MeSH: Aged; Aortic Aneurysm, Abdominal; genetics; metabolism; Female; Gene Expression Profiling; Humans; Male; Middle Aged; Oligonucleotide Array Sequence Analysis; RNA, Long Noncoding; genetics; metabolism
From: Biomedical and Environmental Sciences 2016;29(10):713-723
CountryChina
Language:English
Abstract:
OBJECTIVEThis study is aimed at observing the role of long noncoding RNAs (lncRNAs) in the pathogenesis of abdominal aortic aneurysm (AAA).
METHODSLncRNA and mRNA expression signatures of AAA tissues and normal abdominal aortic tissues (NT) were analyzed by microarray and further verified by Real-time quantitative reverse-transcription PCR (qRT-PCR). The lncRNAs-mRNAs targeting relationships were identified using computational analysis. The effect of lnc-ARG on 5-lipoxygenase (ALOX5) expression was tested in HeLa cells.
RESULTSDifferential expressions of 3,688 lncRNAs and 3,007 mRNAs were identified between AAA and NT tissues. Moreover, 1,284 differentially expressed long intergenic noncoding RNAs and 206 differentially expressed enhancer-like lncRNAs adjacent to protein-coding genes were discerned by bioinformatics analysis. Some differentially expressed lncRNAs and mRNAs between AAA and normal tissue samples were further verified using qRT-PCR. A co-expression network of coding and noncoding genes was constructed based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. In addition, the lnc-ARG located within the upstream of ALOX5 was sorted as a noncoding transcript by analyzing the protein-coding potential using computational analysis. Furthermore, we found that lnc-ARG can decrease the mRNA level of ALOX5 and reactive oxygen species production in HeLa cells.
CONCLUSIONThis study revealed new lncRNA candidates are related to the pathogenesis of AAA.