Characterization and Expression Analysis of Peroxiredoxin Genes in NNK-induced V79 Cells.

Author: Gui Qin SHI ¹ ; Wen Shan ZHOU ¹ ; Meng LI ¹ ; Fei REN ¹ ; Ya Wei HAN ¹
Author Information

1. The College of food and biological engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, China.
Publication Type:Journal Article
MeSH: Animals; Carcinogens; administration & dosage; toxicity; Cell Line; Cell Survival; Cricetinae; Cricetulus; Dose-Response Relationship, Drug; Gene Expression Regulation; drug effects; Nitrosamines; administration & dosage; toxicity; Peroxiredoxins; genetics; metabolism
From: Biomedical and Environmental Sciences 2017;30(3):224-228
CountryChina
Language:English
Abstract: 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent and prevalent nitrosamine procarcinogen found in cigarette smoke. The aim of this work is to study alterations in peroxiredoxin (Prx) expression induced by NNK during carcinogenesis. Characterization of Prx genes from hamster was performed using bioinformatics. V79 cells were induced with different concentrations of NNK (0.1-0.4 mg/mL), and the expression levels of six Prx genes (Prx1-Prx6) were measured by qRT-PCR 24 h following NNK treatment. Prx gene expression was induced by NNK stress, and the highest transcription levels were induced by over 20.42-fold relative to that of the control. NNK induced alterations in Prx expression over the course of lung cancer, which means Prxs may play important roles in ROS detoxification under NNK stress and their functions are complementary.