Expressions of the γ2 chain of laminin-5 and secreted protein acidic and rich in cysteine in esophageal squamous cell carcinoma and their relation to prognosis.
- Author:
Li-Yan XUE
1
;
Shuang-Mei ZOU
;
Shan ZHENG
;
Xiu-Yun LIU
;
Peng WEN
;
Yan-Ling YUAN
;
Dong-Mei LIN
;
Ning LU
Author Information
1. Department of Pathology, Cancer Institute/Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, PR China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
metabolism;
pathology;
Esophageal Neoplasms;
metabolism;
pathology;
Female;
Follow-Up Studies;
Humans;
Laminin;
metabolism;
Lymphatic Metastasis;
Male;
Middle Aged;
Neoplasm Grading;
Neoplasm Invasiveness;
Neoplasm Staging;
Osteonectin;
metabolism;
Survival Rate
- From:Chinese Journal of Cancer
2011;30(1):69-78
- CountryChina
- Language:English
-
Abstract:
Previous studies have shown that the expressions of the γ2 chain of laminin-5 and secreted protein acidic and rich in cysteine (SPARC) play important roles in oncogenesis and the development of carcinoma. To assess the expressions of laminin-5 γ2 chain and SPARC in esophageal squamous cell carcinoma (SCC), and to clarify the prognostic significance of the expressions of laminin-5 γ2 chain and SPARC in esophageal SCC, we detected the expressions of laminin-5 γ2 chain and SPARC in cancer tissue and corresponding normal mucosa from 116 patients with advanced (stages II-IV) esophageal SCC using the tissue microarray-based immunohistochemistry and analyzed the correlation of the expressions with clinicopathologic characteristics and survival. We found that in normal esophageal tissues, laminin-5 γ2 chain was expressed in the basement membrane, whereas in esophageal SCC tissues, laminin-5 γ2 chain was expressed in the cytoplasm of carcinoma cells, with a positive rate of 72.4%. SPARC was not detected in normal esophageal mucosa, but was expressed in stromal fibroblasts in 84.6% of esophageal SCC cases and in cancer cells in 7.8% of esophageal SCC cases. There was a significant correlation between laminin-5 γ2 chain and stromal SPARC expression in esophageal SCC (Spearman's rho=0.423, P<0.001). The expressions of both laminin-5 γ2 chain and stromal SPARC were correlated with survival (P=0.032 and P=0.034, respectively). In stage-II esophageal SCC, the expression of laminin-5 γ2 chain was significantly correlated with survival (P=0.023), while the expression of SPARC was not significantly correlated with survival (P=0.154). Patients with elevated levels of laminin-5 γ2 chain and SPARC expressions had a poorer prognosis than did those lacking elevated levels of laminin-5 γ2 chain expression and/or elevated levels of SPARC expression (P=0.001). In stage-II esophageal SCC, patients with elevated levels of laminin-5 γ2 chain and SPARC expressions had a poorer prognosis (P<0.001). These results suggest that laminin-5 γ2 chain and SPARC may play roles in the progression of esophageal SCC and their simultaneous expression is correlated with poorer prognosis, especially in patients with stage-II SCC.
