Sequence and Structural Analyses of the Complete Genome of Bovine Papillomavirus 2 Genotype Aks-01 Strain from Skin Samples of Cows in Southern Xinjiang, China.
- Author:
Wanqi ZHANG
;
Jianjun HU
;
Shilei YAN
;
Yaojie HUANG
;
Jianping XU
;
Zhongwu HUANG
;
Maoliang ZHENG
;
Ziyan MENG
;
Yuanyuan LI
;
Na WANG
;
Qingqing WANG
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
Bovine papillomavirus 1;
genetics;
Cattle;
China;
Evolution, Molecular;
Female;
Genome, Viral;
genetics;
Genomics;
Genotype;
Molecular Sequence Data;
Oncogene Proteins, Viral;
chemistry;
genetics;
metabolism;
Phylogeny;
Sequence Analysis, DNA;
Skin;
virology
- From:
Chinese Journal of Virology
2015;31(4):370-378
- CountryChina
- Language:Chinese
-
Abstract:
To study the complete genomic sequence, genomic characteristics, and genetic variation of the bovine papillomavirus 2 genotype (BPV-2) Aks-01 strain at the molecular level, genotyping of this strain from the skin samples of cows in southern Xinjiang (China) was first detected by the polymerase chain reaction with FAP59/FAP64 primers. Based on the complete genome of the BPV-2 reference strain, specific primers and sequencing primers were designed, and the complete genome of the Aks-01 strain amplified and sequenced. Sequence analyses showed that genotyping of the Aks-01 strain belonged to BPV-2. The Aks-01 strain had the structural characteristics of BPV-2. The 7944-bp full-length genomic sequence of the Aks-01 strain was compiled using DNAStar™. The sequence of the Aks-01 strain had 98% similarity to the reference strain from GenBank. The Aks-01 strain was most closely related to BPV-1 and BPV-13. BPV-2, BPV-1 and BPV-13 were grouped within the genus Deltapapillomavirus. The Aks-01 strain is the first BPV-2 strain reported in southern Xinjiang.
