Mechanism Underlying Increased Expression of a Member of the Serine/Threonine Kinase Family (Citron kinase) Induced by HIV-1 Infection.
- Author:
Jiwei DING
;
Zeyun MI
;
Jianyuan ZHAO
;
Jinming ZHOU
;
Xiaoyu LI
;
Shan CEN
- Publication Type:Journal Article
- MeSH:
CD4-Positive T-Lymphocytes;
virology;
Cloning, Molecular;
Gene Expression Regulation, Enzymologic;
HEK293 Cells;
HIV-1;
physiology;
Humans;
Intracellular Signaling Peptides and Proteins;
genetics;
Protein-Serine-Threonine Kinases;
genetics;
Up-Regulation;
Virus Replication
- From:
Chinese Journal of Virology
2015;31(4):388-394
- CountryChina
- Language:Chinese
-
Abstract:
Human immunodeficiency virus (HIV)-1 infection changes transcriptional profiles and regulates. the factors and machinery of the host that facilitate viral replication. Our previous study suggested that the serine/threonine kinase citron kinase (citK) promotes HIV-1 egress. To ascertain if HIV-1 infection affects citK expression in primary cells, peripheral blood mononuclear cells were infected with vesicular stomatitis virus G protein (VSV-G)-pseudotyped HIV-1 vector NL4-3-luc viruses, which resulted in remarkably increased expression of citK. citK overexpression led to a more than two-fold increase in HIV-1 production, whereas a significant decrease was observed when citK was depleted in CD4+ T cells. Infection with HIV-1 pseudoviruses induced increases in the mRNA and protein levels of citK by 2. 5- and 2. 7-fold in HEK293T cells, respectively. By cloning the 5-kb promoter of citK into a luciferase reporter system and transfecting the construct into HEK293T cells, enhanced luciferase activity was observed during HIV-1 infection. Taken together, these data demonstrate that HIV-1 infection upregulates citK expression at the transcriptional level, and thereby renders the host more susceptible to invasion by HIV-1.
