Identification of MicroRNA-like Viral Small RNAs from AaeDV.
- Author:
Yanhai WANG
;
Jiang WU
;
Jinbao GU
- Publication Type:Journal Article
- MeSH:
Aedes;
virology;
Animals;
Base Sequence;
Computational Biology;
Densovirinae;
chemistry;
genetics;
metabolism;
MicroRNAs;
chemistry;
genetics;
metabolism;
Molecular Sequence Data;
RNA, Viral;
chemistry;
genetics;
metabolism
- From:
Chinese Journal of Virology
2015;31(5):537-541
- CountryChina
- Language:Chinese
-
Abstract:
Several virus families have been shown to encode microRNAs (miRNAs), which have roles in the infection and replication of viruses in host cells. These virus-encoded miRNAs are identified in double-stranded DNA virus (dsDNA virus) and in several RNA virus families, but not in single-stranded DNA virus (ssDNA virus). We used a bioinformatics approach based on VMir, miRNAFold and MaturePred software to predict virus-encoded miRNA-like small RNAs from the genome of a ssDNA virus: Aedes aegypti densovirus (AaeDV). Northern blotting and stem-loop reverse transcription-polymerase chain reaction (RT-PCR) were used to detect predicted small RNAs. A miRNA-like small RNA termed "AaeDVMD" was identified by stem-loop RT-PCR from predicted candidates. This is the first report demonstrating that a ssDNA virus can encode miRNA-like small RNAs. These data will aid further exploration of the interaction between the AaeDV and its mosquito host.
