Selection and Identification of the Biological Characteristics of a Cold-adapted Genotype G1P8 ZTR-68 Rotavirus by Serial Cold-adapted Passaging.
- Author:
Li XIE
;
Kai MI
;
Jing YE
;
Xianglian NIU
;
Xiaoqin SUN
;
Shan YI
;
Hongjun LI
;
Maosheng SUN
- Publication Type:Journal Article
- MeSH:
Adaptation, Physiological;
China;
Cold Temperature;
Diarrhea;
virology;
Female;
Genotype;
Humans;
Infant;
Male;
Rotavirus;
genetics;
growth & development;
isolation & purification;
physiology;
Serial Passage;
Virus Cultivation;
Virus Replication
- From:
Chinese Journal of Virology
2015;31(5):548-553
- CountryChina
- Language:Chinese
-
Abstract:
We wished to select a cold-adapted genotype G1P[8] ZTR-68 rotavirus (China southwest strain) in MA104 cells for possible use as a live vaccine. ZTR-68 was recovered originally from children with diarrhea. The virus was cultivated at 37 degrees C at the first passage. Then, the cultivation temperature was decreased stepwise by 3 degrees C per eight passages. In total, the virus was passaged 32 times, and cultivation was terminated at 28 degrees C. Biological characteristics of the virus were analyzed during serial passages. There was no difference between the migration patterns of genomic dsRNA segments according to polyacrylamide gel electrophoresis of original and cold-adapted viruses. Infectious and red cell-agglutination titers of cold-adapted virus were lower than those of the parent virus. Also, the virus formed small-size plaques with irregular shapes at 31 degrees C and 28 degrees C. These results suggested that a genetically stable attenuated virus can be obtained through serial cold-adapted passages. Thus, an alternative strategy is provided by cold-adaption for development of attenuated live rotavirus vaccines.
