Sample Preparation and Imaging of Single Adenovirus Particle Using Atomic Force Microscopy in Liquid.
- Author:
Yan LIANG
;
Li CHEN
;
Mariska G M van ROSMALEN
;
Gijs J L WUITE
;
Wouter H ROOS
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
chemistry;
Imaging, Three-Dimensional;
Microscopy, Atomic Force;
methods;
Virion;
chemistry
- From:
Chinese Journal of Virology
2015;31(6):601-606
- CountryChina
- Language:Chinese
-
Abstract:
Atomic force microscopy (AFM), as a sophisticated imaging tool with nanoscale resolution, is widely used in virus research and the application of functional viral particles. To investigate single viruses by AFM in a physiologically relevant environment (liquid), an appropriate surface treatment to properly adhere the viruses to the substrate is essential. Here we discuss hydrophobic treated glass coverslips as a suitable substrate for the adhesion of single adenovirus particle (Adenovirus type 5 F35, Ad5F35) when studied with AFM in liquid. From the high resolution AFM images, the orientation of the adhered virus particles can be distinguished. Furthermore, the particles exhibit the expected height of -90 nm. This illustrates that the viruses adhere to the substrate firmly without large deformations. Hence, the described method works well on (fragile) viruses. The described experimental approach can be widely used for AFM studies in liquid of virus structure and mechanics as well as for investigating the interaction of viruses with cellular receptors.
