Inclusion Bodies are Formed in SFTSV-infected Human Macrophages.
- Author:
Cong JIN
;
Jingdong SONG
;
Ying HAN
;
Chuan LI
;
Peihong QIU
;
Mifang LIANG
- Publication Type:Journal Article
- MeSH:
Cell Line;
China;
Humans;
Inclusion Bodies, Viral;
ultrastructure;
virology;
Macrophages;
ultrastructure;
virology;
Phlebotomus Fever;
virology;
Phlebovirus;
genetics;
physiology;
ultrastructure;
Thrombocytopenia;
virology
- From:
Chinese Journal of Virology
2016;32(1):19-25
- CountryChina
- Language:Chinese
-
Abstract:
The severe fever with thrombocytopenia syndrome virus (SFTSV) is a new member in the genus Phlebovirus of the family Bunyaviridae identified in China. The SFTSV is also the causative pathogen of an emerging infectious disease: severe fever with thrombocytopenia syndrome. Using immunofluorescent staining and confocal microscopy, the intracellular distribution of nucleocapsid protein (NP) in SFTSV-infected THP-1 cells was investigated with serial doses of SFTSV at different times after infection. Transmission electron microscopy was used to observe the ultrafine intracellular structure of SFTSV-infected THP-1 cells at different times after infection. SFTSV NP could form intracellular inclusion bodies in infected THP-1 cells. The association between NP-formed inclusion bodies and virus production was analyzed: the size of the inclusion body formed 3 days after infection was correlated with the viral load in supernatants collected 7 days after infection. These findings suggest that the inclusion bodies formed in SFTSV-infected THP-1 cells could be where the SFTSV uses host-cell proteins and intracellular organelles to produce new viral particles.
