Lentivirus Delivery of the Short Hairpin RNA Targeting NDV P Gene Inhibits Production of the Newcastle Disease Virus in Chicken Embryo Fibroblasts and Chicken Embryos.
- Author:
Shaohua YANG
;
Chuantian XU
;
Lin ZHANG
;
Yanyan HUANG
;
Qinghua HUANG
;
Beixia HU
;
Xiumei ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Chick Embryo;
Chickens;
Down-Regulation;
Fibroblasts;
virology;
Gene Targeting;
Lentivirus;
genetics;
metabolism;
Newcastle Disease;
virology;
Newcastle disease virus;
genetics;
physiology;
Phosphoproteins;
genetics;
metabolism;
Poultry Diseases;
virology;
RNA Interference;
RNA, Small Interfering;
genetics;
metabolism;
Viral Proteins;
genetics;
metabolism;
Virus Replication
- From:
Chinese Journal of Virology
2016;32(1):39-45
- CountryChina
- Language:Chinese
-
Abstract:
Small interfering ribonucleic acid (siRNA)-induced RNA degradation can inhibit viral infection, and has been investigated extensively for its efficacy as antiviral therapy. The potential therapeutic role of lentiviral-mediated short hairpin ribonucleic acid (shRNA) to Newcastle disease virus (NDV) replication in vivo has been explored less often. We constructed two recombinant lentiviral vectors containing shRNA against the phosphoprotein (P) of the NDV, RNAi-341 and RNAi-671. Recombinant shRNA lentivirus vectors were co-transfected into 293T cells, along with helper plasmids, to package the recombinant shRNA lentivirus. Lentivirus-based shRNAs were titrated and transduced into NDV-susceptible chicken embryo fibroblasts (CEFs) and chick embryos. Antiviral activity against the NDV strain was evaluated by virus titration and real-time reverse transcription-polymerase chain reaction. RNAi-341 and RNAi-671 strongly suppressed transient expression of a FLAG-tagged P fusion protein in 293T cells. RNAi-341 and RNAi-671 NDV reduced virus titers by 66.6-fold and 30.6-fold, respectively, in CEFs 16 h after infection. RNAi-341 and RNAi-671 reduced virus titers in specific pathogen-free chick embryos by 99% and 98%, respectively, 48 h after infection. Both shRNAs inhibited accumulation of not only P-gene mRNA, but also nucleocapsid, M-, F-, HN-, and L-gene mRNA. RNAi-341 silenced P-gene mRNA more potently than RNAi-671. These results suggest that shRNAs silencing the P gene had substantial antiviral properties and inhibited NDV replication in CEFs and chick embryos.
