Preparation and Identification of High Immunogenic A/PR/8/34 Maternal Strain HA Protein for Influenza Virus Classical Reassortment.
- Author:
Jing TANG
;
Li XIN
;
Junfeng GUO
;
Wenfei ZHU
;
Heyuan ZHANG
;
Shaohui LANG
;
Dayan WANG
;
Yuelong SHU
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
immunology;
Electrophoresis, Polyacrylamide Gel;
Female;
Hemagglutination Inhibition Tests;
Hemagglutinin Glycoproteins, Influenza Virus;
analysis;
immunology;
Humans;
Influenza A Virus, H1N1 Subtype;
genetics;
immunology;
Influenza, Human;
immunology;
virology;
Rabbits;
Reassortant Viruses;
genetics;
immunology
- From:
Chinese Journal of Virology
2016;32(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
Preparation of maternal strain A/PR/8/34 HA antiserum for influenza virus classical reassortment. A/PR/8/34 virus was digested by bromelain after inactivation and purification. 5%-20% sucrose continuous density gradient centrifugation method was used to purify HA protein. SIRD method was used to select the target protein. SDS-PAGE method was used to identified HA protein. High Immunogenic A/PR/8/34 HA protein was successfully prepared and HI titer reached 10240. High purity HA antiserum was identified by SIRD method. The key reagent in the classical reassortment of influenza virus was prepared, and the complete set of technical methods were explored, which laid the foundation for the independent research and development of seasonal influenza vaccine strains of China.
