Generation of Japanese Encephalitis Virus-like Particle Vaccine and Preliminary Evaluation of Its Protective Efficiency.
- Author:
Yanfang ZHANG
;
Ruikun DU
;
Shaomei HUANG
;
Tao ZHANG
;
Jinliang LIU
;
Bibo ZHU
;
Hualin WANG
;
Fei DENG
;
Shengbo CAO
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
immunology;
Encephalitis Virus, Japanese;
genetics;
immunology;
Encephalitis, Japanese;
immunology;
prevention & control;
virology;
Humans;
Japanese Encephalitis Vaccines;
administration & dosage;
genetics;
immunology;
Mice;
Mice, Inbred BALB C;
Sf9 Cells;
Vaccination;
Vaccines, Virus-Like Particle;
administration & dosage;
genetics;
immunology;
Viral Envelope Proteins;
administration & dosage;
genetics;
immunology
- From:
Chinese Journal of Virology
2016;32(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
The cDNA fragment of JEV prME gene was cloned into the baculovirus shuttle vector (bacmid) to construct a recombinant baculovirus vector, defined as AcBac-prME. Then the recombinant baculovirus Ac-prME was obtained by transfecting Sf9 cells with AcBac-prME. Western blot analysis and immunofluorescence results indicated that both prM and E proteins were efficiently expressed in Sf9 cells. Electron microscopy suggested that prME was assembled into JEV-VLPs. To further evaluate the potential of JEV-VLPs as vaccine, the mice were immunized with JEV-VLPs and then challenged with lethal JEV. The results of mice survival and pathological changes demonstrated that the JEV-VLPs performed complete protection against JEV-P3 strain and relieved pathological changes in the mice brain significant. This study suggest that JEV-VLPs would be a potential vaccine for Japanese encephalitis virus.
