Influence of RNA interference on MSI-2 gene in THP-1 cell and expression of NUMB.
- Author:
Yunfei HUANG
1
;
Qitian MU
1
;
Mengxia YU
1
;
Yungui WANG
1
;
Jie JIN
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; metabolism; Cell Line, Tumor; Cell Proliferation; Down-Regulation; Humans; Membrane Proteins; genetics; metabolism; Nerve Tissue Proteins; genetics; metabolism; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases; metabolism; RNA Interference; RNA, Small Interfering; RNA-Binding Proteins; genetics; metabolism; Transfection
- From: Chinese Journal of Hematology 2015;36(10):858-861
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of small interfering RNA（siRNA）for MSI-2 on the growth, apoptosis and NUMB expression of THP-1 cells.
METHODSThree siRNA for MSI-2 gene was designed and transfected into THP- 1 cells. The cell inhibition, colony formation and apoptosis were determined. The protein expression of NUMB, caspase- 3 and PARP were detected by Western blotting.
RESULTSAfter MSI- 2 expression of THP- 1 cells was down- regulated for 24 hours, cell inhibition of siRNA MSI-2 group was（47.89±7.64）%, obviously higher than that of negative control group（P=0.005）. After 9 days, cell colony count of siRNA MSI-2 group was 7.50±1.53, also lower than that of negative control group（35.75±7.46, P<0.001）. In addition, apoptotic rates of siRNA MSI- 2 group at 24 hours ［（15.22±1.52）%］and 48 hours［（33.83±3.96）%］were significantly higher than those of negative control group（P=0.008 and P=0.001, respectively）. Accordingly, activations of caspase-3 and PARP and increased NUMB were observed in siRNA MSI- 2 group.
CONCLUSIONsiRNA for MSI- 2 gene could increase the expressions of NUMB to inhibit the proliferation and induce apoptosis of THP-1 cells.
