Expression of cMPO in 502 cases of acute myeloid leukemia (AML) and its diagnosis significance in AML subtypes.
- Author:
Lei SHANG
1
;
Xuejing CHEN
1
;
Yuanyuan LI
1
;
Guiqing GUO
1
;
Dashui HE
1
;
Xiaojin CAI
1
;
Bin ZHENG
1
;
Yingchang MI
1
;
Jianxiang WANG
1
;
Kun RU
1
;
Huijun WANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Flow Cytometry; Granulocytes; Humans; Leukemia, Myeloid, Acute; classification; genetics; Peroxidase; genetics
- From: Chinese Journal of Hematology 2015;36(11):906-911
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the myeloperoxidase (cMPO) expression pattern by flow cytometry (FCM) in patients with acute myeloid leukemia (AML) and its role in classifying AML.
METHODSEight- color multiparametric FCM with CD45/SSC gating was used to determine the cMPO expression in 502 AML patients.
RESULTSThe positive rate of cMPO in all patients was 58.0%, in which the proportion of normal positivity, dim positivity and partial positivity was 21.5%, 34.1% and 2.4%, respectively. The remaining case (42.0%) were all negative. In AML with t (15;17）(q22;q12)/PMLRARα, the positive rate was the highest (100%) and the intensity was similar to that of the normal granular leukocytes, followed by AML with t (8;21（q22;q22/RUNX1-RUNX1T1, the positive rate was 91.4% and the intensity was mostly dim. AML with minimal differentiation and acute megakaryoblastic leukemia were all cMPO negative. The positive rates of cMPO in the remaining subtypes were between 22.7% and 76.2%.
CONCLUSIONThe positive rate and intensity of cMPO were significantly different among different subtypes of AML.
