Analysis of KIR3DL1 expression levels on 92 cases of normal donors for hematopoietic stem cells transplant.
- VernacularTitle:KIR3DL1基因在92例造血干细胞供者中的表达
- Author:
Jing ZHANG
1
;
Miao WANG
1
;
Xiaojing BAO
1
;
Xiaojin WU
1
;
Huifen ZHOU
1
;
Huanhuan ZHANG
1
;
Depei WU
1
;
Jun HE
1
Author Information
- Publication Type:Journal Article
- MeSH: Genotype; HLA-B Antigens; genetics; Haplotypes; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells; Humans; Killer Cells, Natural; metabolism; Ligands; Receptors, KIR3DL1; genetics; Tissue Donors
- From: Chinese Journal of Hematology 2015;36(12):994-998
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study KIR3DL1 expression level on NK cell surface of normal donors for hematopoietic stem cell transplantation（HSCT）.
METHODSNinety- two donors were performed by using of KIR genotyping, HLA high resolution genotyping and KIR3DL1 expression level using sequencebased testing（SBT）, PCR- sequence specific primer（SSP）and flow cytometry methods.
RESULTSIn 92 donors, the frequencies of KIR-A/A, Bx1, Bx2 for common genotypes were 46.74%（43/92）, 18.48% （17/92）and 9.78%（9/92）respectively（P<0.001）; KIR-A, B1, B2, B3 for common KIR haplo-type were 70.33%（128/182）, 10.99%（20/182）, 7.14%（13/182） and 4.39%（8/182） respectively（P<0.001）; the frequencies of HLA-BW4/BW4, HLA-BW4/BW6, BW6/BW6 ligands were 13.79%, 67.81% and 18.39% respectively（P<0.001）. KIR3DL1 middle expression level among haplo- type KIR- A/A and KIR- Bx, KIR-B/B were 18.77%（3.11%-49.24%）, 13.14%（1.70%-63.32%）and 0.37%（0.20%-2.60%）respectively （P<0.05）. KIR3DL1 expression level［18.77%（3.11%-49.24%）］in haplo-type KIR-A/A was higher than haplo-type KIR-Bx at the same time did not express 2DL2 group［11.20%（3.50%-36.08%）］（P=0.019）. KIR3DL1 expression level in recognition group（HLA-BW4 positive group）［17.61%（1.40%-49.24%）］ was higher than KIR3DL1 unrecognized group（HLA-BW4 negative group）［10.60%（3.50%-18.56%）］ （P=0.006）.
CONCLUSIONThe expression levels of KIR3DL1 in different KIR genotypes, haplotypes and HLA ligands were statistically significance.
