Evaluation of two anti-hepatitis E virus IgM kits.
- Author:
Sheng-Xiang GE
1
;
Ying-Jie ZHENG
;
Qing-Shun GUO
;
Jun ZHANG
;
Qing-Wu JIANG
;
Mun-Hon NG
;
Ning-Shao XIA
Author Information
- Publication Type:Journal Article
- MeSH: Diagnosis, Differential; Hepatitis E; diagnosis; immunology; Humans; Immunoglobulin M; blood; immunology; Reagent Kits, Diagnostic; Sensitivity and Specificity
- From: Biomedical and Environmental Sciences 2007;20(6):512-515
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate two commercial anti-hepatitis E virus (HEV) IgM kits used for differential diagnosis of acute enteric viral hepatitis.
METHODSThe kit for IgM capture assay, was produced with a recombinant HEV structural protein protecting primates against experimental infection by different HEV genotypes, while the other kit for indirect ELISA was produced with recombinant structural proteins from different HEV genotypes. The serum specimens were taken from 241 cases with a confirmed or presumptive diagnosis of hepatitis A and 74 cases with a confirmed or presumptive diagnosis of hepatitis E.
RESULTSThe sensitivity and specificity of the IgM capture assay kit were 97% and 100%, respectively, and the corresponding values for the other kit were 70% and 78%, respectively.
CONCLUSIONThe IgM capture assay kit has higher sensitivity and specificity in diagnosing acute enteric viral hepatitis E.
