Effects of nonylphenol on brain gene expression profiles in F1 generation rats.
- Author:
Yin-Yin XIA
1
;
Ping ZHAN
;
Yang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; drug effects; metabolism; Female; Gene Expression Profiling; Male; Phenols; pharmacology; Rats; Rats, Sprague-Dawley
- From: Biomedical and Environmental Sciences 2008;21(1):1-6
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the effects of nonylphenol on brain gene expression profiles in F1 generation rats by microarray technique.
METHODSmRNA was extracted from the brain of 2-day old F1 generation male rats whose F0 female generation was either exposed to nonylphenol or free from nonylphenol exposure, and then it was reversely transcribed to cDNA labeled with cy5 and cy3 fluorescence. Subsequently, cDNA probes were hybridized to two BiostarR-40S cDNA gene chips and fluorescent signals of cy5 and cy3 were scanned and analyzed. Results Two genes were differentially down-regulated.
CONCLUSIONNonylphenol may disturb the neuroendocrine function of male rats when administered perinatally.
