Immunological effect of PM2.5 on cytokine production in female Wistar rats.
- Author:
Ning-Hua HUANG
1
;
Qin WANG
;
Dong-Qun XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchoalveolar Lavage Fluid; Cytokines; biosynthesis; genetics; metabolism; Enzyme-Linked Immunosorbent Assay; Female; Macrophages, Alveolar; metabolism; Particle Size; Phagocytosis; RNA, Messenger; genetics; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction
- From: Biomedical and Environmental Sciences 2008;21(1):63-68
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the immunological effect of PM2.5 on cytokine production in female Wistar rats.
METHODSFemale Wistar rats were given 0.3 mg, 0.75 mg, 2 mg, 5 mg of PM2.5 per 0.5 mL saline, respectively. Saline was used as the negative control. TNF-alpha and IL-6 levels in the branchoalveolar lavage were measured by ELISA, and mRNA expression levels in lung tissue were detected by RT-PCR. Alveolar macrophages were collected for testing phogacytic function.
RESULTSExposure to PM2.5 stimulated TNF-alpha production in a dose-dependent manner (P < 0.05), However, no statistically significant difference was found. No time-dependent change in TNF-a and IL-6 production was found. TNF-alpha and IL-6 mRNA expressions were induced by PM2.5-exposure. The phagocytic rate (PR) was significantly decreased by PM2.5 treatment.
CONCLUSIONPM2.5 exposure increases inflammation response of the lung in a dose-dependent manner. Moreover, tissue injury induced by PM2.5 may be related to altered production of cytokines. PMz2.5 may impair the phagocytic activity of alveolar macrophages.
