Reversal of apoptotic resistance by Lycium barbarum glycopeptide 3 in aged T cells.
- Author:
Long-Guo YUAN
1
;
Hong-Bin DENG
;
Li-Hui CHEN
;
Dian-Dong LI
;
Qi-Yang HE
Author Information
- Publication Type:Journal Article
- MeSH: Aging; immunology; Animals; Apoptosis; drug effects; Fas Ligand Protein; immunology; Glycopeptides; pharmacology; Interferon-gamma; genetics; immunology; Interleukin-10; genetics; immunology; Lycium; chemistry; Mice; Mice, Inbred C57BL; Proto-Oncogene Proteins c-bcl-2; immunology; RNA, Messenger; immunology; T-Lymphocytes; drug effects; immunology
- From: Biomedical and Environmental Sciences 2008;21(3):212-217
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study whether Lycium barbarum glycopeptide 3 (LBGP3) affects T cell apoptosis in aged mice.
METHODSLBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic "sub-G1 peak" was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-gamma and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FLIP, FasL, and Bcl-2 were determined by Western blotting.
RESULTSLBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide. Treatment with 200 microg/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T cells. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FLIP, and up-regulating the expression of FasL.
CONCLUSIONLycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules.
