MicroRNA expression profiles and miR-10a target in anti-benzoa pyrene-7, 8-diol-9, 10-epoxide-transformed human 16HBE cells.
- Author:
Yue-Lan SHEN
1
;
Yi-Guo JIANG
;
Anne R GREENLEE
;
Lan-Lan ZHOU
;
Lin-Hua LIU
Author Information
- Publication Type:Journal Article
- MeSH: 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; toxicity; Carcinogens; toxicity; Cell Transformation, Neoplastic; chemically induced; genetics; metabolism; Cells, Cultured; Gene Expression Profiling; Homeodomain Proteins; genetics; metabolism; Humans; MicroRNAs; metabolism; Oligonucleotide Array Sequence Analysis; RNA, Messenger; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; genetics; metabolism
- From: Biomedical and Environmental Sciences 2009;22(1):14-21
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo screen miRNA profiles of malignantly transformed human bronchial epithelial cells, 16HBE-T, induced by anti-benzo[a]pyrene-trans-7,8-diol-9,10-epoxide (anti-BPDE), and to analyze putative miR-10a targets in 16HBE-T.
METHODSA novel microarray platform was employed to screen miRNA profiles of 16HBE-T cells transformed by anti-BPDE. Microarray data for miR-10a and miR-320 were validated using quantitative real time polymerase chain reaction (QRT-PCR). The expression of a putative target for miR-10a, HOXA1, was analyzed by reverse transcription polymerase chain reaction (RT-PCR) and QRT-PCR.
RESULTSIn comparison with the vehicle-treated cells (16HBE-N), 16HBE-T exhibited differential expression of 54 miRNAs, in which, 45 were over-expressed and 9 were down-regulated. The five most highly expressed miRNAs were miR-494, miR-320, miR-498, miR-129, and miR-106a. The lowest expressed miRNAs were miR-10a, miR-493-5p, and miR-363*. Three members of miR-17-92 cluster, miR-17-5p, miR-20a, and miR-92, showed significantly higher abundance in 16BHE-T as miR-21, miR-141, miR-27a, miR-27b, miR-16 and miRNAs of the let-7 family. The putative target for miR-10a, HOXA1 mRNA was up-regulated 3-9-fold in 16HBE-T, as compared with 16HBE-N.
CONCLUSIONThe findings of the study provide information on differentially expressed miRNA in malignant 16HBE-T, and also suggest a potential role of these miRNAs in cell transformation induced by anti-BPDE. HOXA1 is similarly up-regulated, suggesting that miR-10a is associated with the process of HOXA 1-mediated transformation.
